Tetrameric UvrD helicase is located at the E. coli replisome due to frequent replication blocks
The sister paper to our study on the Rep helicase. Using dual colour live cell single-molecule fluorescence microscopy, we investigated how E.coli’s other accessory helicase, UvrD, functions. We found that differently to Rep, UvrD, is not recruited to the replisome by a specific replisome partner. Rather the replisome encounters so many other blocks to replication which recruit UvrD that there is almost always UvrD present at the replication fork.
Wollman Lab 